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The ORF were inserted into the shuttle vectors using NEBuilder HiFi DNA Assembly mix (New England Biolabs) utilising the 15 bp homology arms to the 5 and 3 end of the F11 or B8 insertion loci

10.1097/MED.0b013e32835edb32 149 SchultesB.OltmannsK

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However, both ROS and antioxidants have dual roles: ROS can induce cancer cell death, and some antioxidants may paradoxically promote tumour growth by reducing high levels of naturally occurring ROS in cancer cells, which would otherwise damage and kill the cells